ABSTRACT
Objective:
Carotid plaques are divided into calcified, mixed and fatty types according to the morphological ultrastructure. Increased risk of rupture, thromboembolism and stroke are more pronounced in mixed and fatty carotid plaques due to composition and instability.
The present study aimed to assess the role of monocyte to high-density lipoprotein cholesterol (HDL-C) ratio (MHR) to predict mixed and fatty plaques in patients with intermediate carotid artery stenosis.
Method:
A total of 223 asymptomatic patients who had 50-70% stenosis in the carotid artery were included in this retrospective cohort study. Patients were assigned into three groups based on plaque morphology: 94 with calcified, 71 with mixed and 58 with fatty plaques. Groups were compared in terms of MHR. Predictive role of MHR for mixed and fatty plaque was investigated.
Results:
MHR was significantly higher in the mixed and fatty plaque groups (16.951±5.935 vs 20.181±9.405 vs 11.200±4.126, p<0.001). It, with a cut off of 13.61, had 71.8% sensitivity and 56.6% specificity for the prediction of mixed plaque [area under the curve (AUC): 0.645, 95% confidence interval (CI): 0.571-0.718, p=0.001]. Moreover, MHR, with a cut off of 14.40, had 77.6% sensitivity and 62.4% specificity for the prediction of fatty plaque (AUC: 0.746, 95% CI: 0.675-0.818, p<0.001). Multivariate regression analysis showed MHR was a significant independent predictor of mixed [odds ratio (OR): 1.230, p<0.001] and fatty (OR: 1.364, p<0.001) plaques in intermediate carotid artery stenosis, after adjusting for other risk factors.
Conclusion:
MHR plays a favorable role for the prediction of mixed and fatty plaques in asymptomatic intermediate carotid artery stenosis.
Introduction
Atherosclerosis is identified as a major cause up to 20-30% of all ischemic strokes in the world (1). The pathogenesis is multifactorial involving inflammation and immune mechanisms (2). Rupture of atherosclerotic plaque causes the activation of coagulation, aggregation of platelets and thrombus formation. Composition should be taken into consideration rather than stenosis severity while interpreting vulnerable plaque (3). Plaques that constitute high triglyceride and low density lipoprotein (LDL) have higher tendency to rupture.
Monocytes are key players of innate immunity. They release proinflammatory cytokines in all stages of inflammation (4,5). Atherosclerotic process from the onset to rupture is mediated by these cytokines (6,7). Monocytes ingest lipoprotein and convert to foam cells. Subsequently, they become main cellular component of atherosclerotic plaque.
Monocyte has a proinflammatory effect, while high-density lipoprotein (HDL) acts as an anti-inflammatory molecule that antagonizes this effect. HDL alleviates the deleterious effect of monocytes by reversing cholesterol transport in the plaque (8,9). Therefore, monocyte/HDL-cholesterol ratio (MHR) is a novel parameter used to evaluate the inflammatory condition. MHR is strikingly high among patients with cardiovascular diseases (10) and stroke (11). Additionally, it plays a strong predictive role for the prognosis after stroke (11).
Carotid plaques are divided into calcified, mixed and fatty types based on morphology detected by computed tomography angiography (CTA) (12). Mixed and fatty plaques have thin fibrous cap and high lipid content. They are unstable and easily ruptured causing transient ischemic attack and stroke (13).
Severity of stenosis and symptoms are two main factors that determine the risk of stroke in carotid artery (14). According to current guidelines (15), two situations requiring intervention are as follows: 1) Symptomatic patients with at least 50% carotid artery stenosis, 2) Asymptomatic patients with at least 70% carotid artery stenosis. There is a gray zone including asymptomatic patients with intermediate (50-70%) carotid artery stenosis. Identification of high risk individuals with intermediate carotid artery stenosis for developing symptoms is crucial. It may be beneficial to construct algorithms by adding MHR and plaque morphology to predict absolute risk. The relationship between these parameters remains to be elucidated. Hence, we hypothesize that MHR plays a strong role for the prediction of mixed and fatty plaques in asymptomatic intermediate carotid stenosis. The present study compares fatty, mixed and calcified plaques that cause asymptomatic intermediate carotid artery stenosis with respect to MHR.
Materials and Methods
Study Population
We retrospectively reviewed the medical records of 305 patients, who were admitted to the cardiology outpatient clinic with a 50-70% stenosis in the carotid artery between 2013 and 2015. Patients with any of the followings were excluded: ischemic or non-ischemic stroke, Acute Coronary syndrome, previous cardiac surgery, known coronary artery disease, atrial fibrillation, concomitant severe valvular disease, heart failure, cardiomyopathy, congenital heart defects, renal or hepatic disease, hematological disorders, malignancy and acute or chronic inflammatory disorders. Finally, 223 consecutive patients with asymptomatic intermediate carotid stenosis were enrolled in the study. Patients were divided into three groups as follows: 1) Ninety-four patients with calcified plaque, 2) Seventy-one patients with mixed plaque, 3) Fifty-eight patients with fatty plaque. Carotid stenosis and morphology were evaluated through CTA in each patient. Evaluation of each patient was performed by a multidisciplinary team including radiologist, neurologist, cardiologist and cardiothoracic surgeon. Local ethics comitte approved the study with the number 3.841 on 06.03.2020. Declaration of Helsinki were taking into consideration while conducting the study.
Statistical Analysis
Mean ± standard deviation were used to assess continuous variables and number and percentages were used to assess categorical variables. Test of normality was performed either Shapiro-Wilk test or Kolmogorov-Smirnov test. Student t-test was the test of choice to investigate differences between continuous variables with normal distribution. If the distribution was not normal for continuous variables, Mann-Whitney U test were performed. Chi-square test was used to assess difference between categorical variables. Sensitivity and specificity analysis were conducted with the receiver-operating characteristics (ROC) curve. Additionally, variables that had predictive value for mixed and fatty plaques in univariate analysis were integrated into multivariate logistic regression to determine statistical significance. A two sided p-value which was smaller than 0.05, was considered statistically significant.
Results
Baseline demographical characteristics and clinical, laboratory data of 223 patients (94 calcified, 71 mixed, 58 fatty) were summarized in Table 1. Fatty plaque group were younger (64.98±7.812 vs 71.31±10.683 vs 72.63±8.257, p<0.001) and smoking (41.4 vs 23.9 vs 20.2%, p=0.013) was more cammon among this group. Additionally white blood cell count (8.899±2.567 vs 8.362±1.959 vs 7.314±1.667×103/L, p<0.001), neutrophil count (5.861±2.164 vs 5.139±1.857 vs 3.938±1.241×103/L, p<0.001) and monocyte count (0.725±0.230 vs 0.685±0.168 vs 0.552±0.177×103/L, p<0.001) were higher in fatty plaque group.
Total cholesterol (188.36±42.791 vs 198.06±43.089 vs 211.71±40.230 mg/dL, p=0.003) and LDL (116.97±34.816 vs 120.89±33.450 vs 130.05±31.044 mg/dL, p=0.041) were lower in fatty plaque group due to more statin usage (25.9%). HDL (38.52±8.923 vs 42.77±10.469 vs 49.94±7.377 mg/dL, p<0.001) was significantly lower in fatty plaque group, respectively.
There was no difference in terms of antiaggregant and statin usage between three groups (Table 2). Few patients were taking acetylsalicylic acid (36.2 vs 28.2 vs 24.5%, p=0.300) and clopidogrel (5.2 vs 7 vs 7.4%, p=0.857) in each group.
Monocyte to high-density lipoprotein cholesterol ratio was found to be higher in fatty and mixed groups (20.181±9.405 vs 16.951±5.935 vs 11.200±4.126, p<0.001). Sensitivity and specificity analysis were conducted with the ROC curve and the respective areas under the curve (AUCs) were used to investigate the predictive value of MHR for prediction of mixed and fatty plaques (Figures 1,2). MHR had 71.8% sensitivity and 56.6% specificity for prediction of mixed plaque [AUC: 0.645, 95% confidence interval (CI): 0.571-0.718, p=0.001]. Moreover MHR had 77.6% sensitivity and 62.4% specificity for prediction of fatty plaque (AUC: 0.746, 95% CI: 0.675-0.818, p<0.001).
Multivariate regression analysis showed MHR was a significant independent predictor of both mixed [odds ratio (OR): 1.230, p<0.001] and fatty (OR: 1.364, p<0.001) plaques in asymptomatic patients with intermediate carotid artery stenosis, after adjusting for other risk factors (Table 3,4).
Discussion
The main findings of the study were as follows: 1) A raised MHR was found to be significantly higher in asymptomatic 50-70% carotid artery stenosis with mixed and fatty plaques, 2) MHR had moderate sensitivity and specificity to predict mixed and fatty plaques and 3) MHR was found to be a significant independent predictor for mixed and fatty plaques in patients with intermediate carotid artery disease, after adjusting for other risk factors in multivariate analysis.
Atherosclerosis is the main pathological finding in patients with ischemic heart disease and cerebrovascular disease (16). Fibrous cap and necrotic center are two layers of atherosclerotic plaque. Foam cells derived from macrophages are the main cellular component and cholesterol crystals are the main acellular component of the plaque. Monocytes and macrophages provoke atherosclerotic process via cytokine secretion and foam cell formation. Cytokines activate all stages of atherosclerosis, including onset, progression, rupture and thrombus formation (17). Total amount of inflammatory markers were found to have a predictive role for subsequent cardiovascular disease (18,19). Moreover, monocyte count as a progenitor of foam cells was shown to be a predictor of new plaque formation (20).
High density lipoprotein, as an antiatherosclerotic molecule, reverses cholesterol transport from the plaque and inhibits activation, adhesion and infiltration of monocytes (8). Murphy et al. (7) postulated in their study that HDL exerted its effect through CD11b molecule on the surface of monocytes. CD11b is responsible for binding to endothelial cells and migrating from vascular wall.
Monocyte/high density lipoprotein ratio emerges as a combination of these two parameters and reflects the underlying inflammatory process. Previous studies demonstrated that MHR was useful for the diagnosis of subclinical carotid atherosclerosis in diabetics (21) and had a prognostic role for patients with coronary artery disease undergoing percutaneous coronary intervention (22).
Stenosis severity and plaque morphology were detected by CTA in this study. Calcified, mixed and fatty plaque morphology in asymptomatic patients with intermediate carotid stenosis were compared in terms of MHR. Previous studies reported calcified plaque constituted higher smooth muscle and lower intraplaque hemorrhages and fatty content. Hence, this plaque was very stable and was more frequently found in asymptomatic patients compared to patients who had ischemic cerebrovascular event (23). Increased risk of rupture and thromboembolism were demonstrated with fatty plaques in the same study (23). Another study by Ahmadi et al. (24) found an additional prognostic role of mixed and fatty plaques in symptomatic with nonobstructive coronary artery disease.
Identification of high risk individuals with intermediate carotid artery stenosis for developing symptoms is crucial. Morphological ultrastructure could give substantial information. Although there are studies comparing the morphological status of plaques causing asymptomatic intermediate carotid artery stenosis in terms of inflammatory parameters (22), no clinical study in the literature has evaluated the predictive value of MHR for this purpose. The relation between mixed and fatty plaques and novel inflammatory markers should be definitely set. Our study showed that MHR had a strong predictive role for mixed and fatty carotid plaques in the carotid artery. Taken together with close relationship of these plaques with other inflammatory markers reported in previous studies (25), mixed and fatty plaques are very unstable and put the patient at high risk category. Few patients were taking antiaggregant and statin in our study. Aggressive medical treatment including high dose statins and antiaggregants may be initiated earlier in asymptomatic intermediate carotid stenosis with mixed and fatty plaque morphology. Additionally, early invasive strategy could be taken into consideration for fatty plaques.
Study Limitations
Some limitations of our study are as follows. It was a single center study and performed in the small population. Serial MHR changes were not evaluated because we only measured MHR at baseline. Due to the lack of registration, no comparison was made between inflammatory marker levels such as C-reactive protein with MHR. Moreover, not all comorbidities and environmental factors that might affect inflammatory markers were taken into account.
Conclusion
Monocyte/high density lipoprotein ratio is a marker of inflammation and atherosclerosis. Increased MHR may be one of the factors associated with the presence of fatty and mixed carotid artery plaques that cause asymptomatic intermediate stenosis.
References
e Fernandes JF, et al. ESVS guidelines. Invasive treatment for carotid stenosis: indications, techniques. Eur J Vasc Endovasc Surg 2009;37(4 Suppl):1-19.